HDX-MS Method Development
Conference Year
2024
Abstract
Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful tool in molecular biology for studying protein structure and dynamics. This study aimed to develop an optimized HDX workflow using bovine cytochrome C (Cyto. C) as a test standard. Front end chromatography was set up to achieve in-line pepsin digestion and fast separation of the resulting peptides at 0℃. Mass spectra were analyzed using HDExaminer software to identify mass spectral shifts of peptic peptides in the deuterated Cyto. C and to calculate the corresponding percent deuterium uptake. This provided experimental evidence about the protein's structure and dynamics.
Primary Faculty Mentor Name
Ying Wai Lam
Graduate Student Mentors
Sydney Cohn Guthrie
Status
Undergraduate
Student College
College of Arts and Sciences
Program/Major
Biochemistry
Primary Research Category
Life Sciences
HDX-MS Method Development
Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful tool in molecular biology for studying protein structure and dynamics. This study aimed to develop an optimized HDX workflow using bovine cytochrome C (Cyto. C) as a test standard. Front end chromatography was set up to achieve in-line pepsin digestion and fast separation of the resulting peptides at 0℃. Mass spectra were analyzed using HDExaminer software to identify mass spectral shifts of peptic peptides in the deuterated Cyto. C and to calculate the corresponding percent deuterium uptake. This provided experimental evidence about the protein's structure and dynamics.