Characterization of protein-protein interactions between subunits of the Retriever complex in Arabidopsis thaliana
Conference Year
2024
Abstract
Endomembrane tra9icking is required for many aspects of plant growth. Studies have identified a protein complex known as Retriever that functions in recycling integral plasma membrane proteins (McNally et al., 2017). Two putative retriever interacting proteins, CCDC22 and CCDC93, are required for root and root hair growth in plants (Lewis et al., 2022). To test protein interactions, we are using a split-YFP assay in which successful interactions in planta will result in reconstitution of a YFP signal. Initial studies will focus on whether CCDC22 and CCDC93, two proteins essential for retriever function in humans, form hetero- and/or homodimers in plants.
Primary Faculty Mentor Name
Mary Tierney
Status
Graduate
Student College
College of Agriculture and Life Sciences
Program/Major
Plant Biology
Primary Research Category
Life Sciences
Characterization of protein-protein interactions between subunits of the Retriever complex in Arabidopsis thaliana
Endomembrane tra9icking is required for many aspects of plant growth. Studies have identified a protein complex known as Retriever that functions in recycling integral plasma membrane proteins (McNally et al., 2017). Two putative retriever interacting proteins, CCDC22 and CCDC93, are required for root and root hair growth in plants (Lewis et al., 2022). To test protein interactions, we are using a split-YFP assay in which successful interactions in planta will result in reconstitution of a YFP signal. Initial studies will focus on whether CCDC22 and CCDC93, two proteins essential for retriever function in humans, form hetero- and/or homodimers in plants.