The role of the Type VI Secretion System (T6SS) in Providencia alcalifaciens.
Conference Year
2024
Abstract
The Providencia genus is a set of emerging, opportunistic pathogens. The genome of P. alcalifaciens 205/92 was found to contain a type VI secretion system (T6SS). The role and functionality of the T6SS in P. alcalifaciens has not been determined. To better understand the conservation of the T6SS across the Providencia genus, a bioinformatic analysis was performed, suggesting that the T6SS proteins are conserved throughout the Providencia genus. We compared P. alcalifaciens wild type bacteria with the ∆tssC and ∆clpV mutants for: (i) colonization of mammalian cells, and (ii) bacterial killing. Our studies indicate that the T6SS in P. alcalifaciens has neither anti-prokaryotic, nor anti-eukaryotic, properties.
Primary Faculty Mentor Name
Leigh Knodler
Graduate Student Mentors
Jean Celli, Emilia Norberg
Status
Undergraduate
Student College
College of Agriculture and Life Sciences
Program/Major
Molecular Genetics
Primary Research Category
Life Sciences
The role of the Type VI Secretion System (T6SS) in Providencia alcalifaciens.
The Providencia genus is a set of emerging, opportunistic pathogens. The genome of P. alcalifaciens 205/92 was found to contain a type VI secretion system (T6SS). The role and functionality of the T6SS in P. alcalifaciens has not been determined. To better understand the conservation of the T6SS across the Providencia genus, a bioinformatic analysis was performed, suggesting that the T6SS proteins are conserved throughout the Providencia genus. We compared P. alcalifaciens wild type bacteria with the ∆tssC and ∆clpV mutants for: (i) colonization of mammalian cells, and (ii) bacterial killing. Our studies indicate that the T6SS in P. alcalifaciens has neither anti-prokaryotic, nor anti-eukaryotic, properties.