Establishing and Streamlining Workflows for High-pH Reversed-Phase HPLC Fractionation For Proteomics Profiling
Conference Year
2024
Abstract
High pH reversed phase HPLC fractionation, when coupled to mass spectrometry, is a useful method for covering nearly the entire proteome. This project aimed at establishing such a workflow for the UVM proteomics core to use for future collaborations with universities across the country. A previously homogenized and digested mouse brain was fractionated with traditional methods, and ran on the mass spectrometer. Proteomics based software was then used to determine mouse brain proteome coverage, and total protein count. This was then compared back to samples run via high pH reversed phase HPLC which should display greater proteome coverage.
Primary Faculty Mentor Name
Ying Wai Lam
Graduate Student Mentors
Sydney Cohn-Guthrie
Status
Undergraduate
Student College
College of Arts and Sciences
Program/Major
Biology
Primary Research Category
Life Sciences
Establishing and Streamlining Workflows for High-pH Reversed-Phase HPLC Fractionation For Proteomics Profiling
High pH reversed phase HPLC fractionation, when coupled to mass spectrometry, is a useful method for covering nearly the entire proteome. This project aimed at establishing such a workflow for the UVM proteomics core to use for future collaborations with universities across the country. A previously homogenized and digested mouse brain was fractionated with traditional methods, and ran on the mass spectrometer. Proteomics based software was then used to determine mouse brain proteome coverage, and total protein count. This was then compared back to samples run via high pH reversed phase HPLC which should display greater proteome coverage.