The impact of modification in metabolism: A focus on MDH1
Abstract
The cytoplasmic enzyme, Malate Dehydrogenase MDH1, contributes to the cellular metabolism and generation of energy. MDH has to be tightly regulated to respond to cellular needs. One type of regulation mechanism is the post-translational modification (PTM) of enzymes, such as ADP-ribosylation. Two amino acids in MDH1 have been identified as ADP-ribosylated by mass spectrometry, but the role of this PTM is unknown. Using in vitro approaches, we are assessing how the absence of ADP-ribosylated amino acids impacts the enzyme’s activity. This work helps us gain a better understanding of the regulatory role of PTM on MDH1 and metabolism.
Primary Faculty Mentor Name
Delphine Quenet
Status
Undergraduate
Student College
College of Arts and Sciences
Program/Major
Biology
Primary Research Category
Life Sciences
The impact of modification in metabolism: A focus on MDH1
The cytoplasmic enzyme, Malate Dehydrogenase MDH1, contributes to the cellular metabolism and generation of energy. MDH has to be tightly regulated to respond to cellular needs. One type of regulation mechanism is the post-translational modification (PTM) of enzymes, such as ADP-ribosylation. Two amino acids in MDH1 have been identified as ADP-ribosylated by mass spectrometry, but the role of this PTM is unknown. Using in vitro approaches, we are assessing how the absence of ADP-ribosylated amino acids impacts the enzyme’s activity. This work helps us gain a better understanding of the regulatory role of PTM on MDH1 and metabolism.
