Negative regulation of hypoxia-inducible factor (HIF-1α) by the transcription factor RUNX1 in breast cancer cells
Conference Year
January 2022
Abstract
Loss of the transcription factor RUNX1 leads to breast cancer cell growth and expression of hypoxia-regulated genes. Our hypothesis is that RUNX1 is a negative regulator of hypoxia-inducible factor-1 (HIF-1α). MCF10A breast cancer cells containing degron-tagged RUNX1 were used to measure effects of RUNX1 degradation. Nuclear fractions were analyzed by Western blot and probed using antibodies for HIF-1α, RUNX1, and a histone control. Although RUNX1 degradation was successful, no significant change in HIF-1α stabilization in normoxia was observed at 4 or 24 hours. Because HIF-1α has a short half-life in normoxia, future experiments will test the effect at additional timepoints.
Primary Faculty Mentor Name
Karen M Lounsbury
Faculty/Staff Collaborators
Andrew Fritz, Gary Stein
Status
Undergraduate
Student College
College of Arts and Sciences
Program/Major
Biochemistry
Primary Research Category
Biological Sciences
Negative regulation of hypoxia-inducible factor (HIF-1α) by the transcription factor RUNX1 in breast cancer cells
Loss of the transcription factor RUNX1 leads to breast cancer cell growth and expression of hypoxia-regulated genes. Our hypothesis is that RUNX1 is a negative regulator of hypoxia-inducible factor-1 (HIF-1α). MCF10A breast cancer cells containing degron-tagged RUNX1 were used to measure effects of RUNX1 degradation. Nuclear fractions were analyzed by Western blot and probed using antibodies for HIF-1α, RUNX1, and a histone control. Although RUNX1 degradation was successful, no significant change in HIF-1α stabilization in normoxia was observed at 4 or 24 hours. Because HIF-1α has a short half-life in normoxia, future experiments will test the effect at additional timepoints.